Chibao Huang, Guanglian Zhao, Xiaonan Liu, Daohai Zhang, Tingxiang Yuan and Yang Liu
A novel two-photon fluorescence probe for Hg2+ derived from bis(styryl)terephthalonitrile as a two-photon fluorophore and bis [2-(2-hydroxyethyl sulfanyl) ethyl] amino group (ionophore) as a novel Hg2+ ligand was developed. The probe possesses small molecule size, large two-photon absorption cross-section (1067 GM) in H2O, noncytotoxic effect, long-wavelength emission at 588 nm, large Stokes shift (121 nm), excellent photostability, high water-solubility, good cell-permeability, and pH-insensitivity in the biologically relevant range. The probe can selectively detect Hg2+ ions in live cells and living tissues without interference from other metal ions and the membrane-bound probes, and its quenching constant (Ksv TP) is 8.73 × 105 M-1.
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