Tesfaye Belachew*
A total of 300 local zebu lactating cows of small holders farmers around Debrezeit were examined to determine the prevalence of mastitis with associated risk factor, isolate bacterial pathogens involved and its antimicrobial susceptibility profiles. Clinical prevalence was determined through examination of abnormalities of milk and udder and California Mastitis Test (CMT) were used for determining subclinical mastitis. Bacterial culture and Agar disc diffusion was used for isolation and antibiotic susceptibility test. Based on the result out of 18 (6%) positive samples, 2 (0.7%) were clinical and the other 16 (5.3%) were sub-clinical mastitis. Among the potential risk factors considered, there was significant difference (P<0.05) between semi-intensive and extensive farming system. All positive samples were positive for aerobic bacteria. The bacterial species isolated were CNS and Micrococcusspecies 22.2%, Staphylococcus aureus 16% Staphylococcus epidermis and Mycoplasma species 11.1%, Enterococcus species, Streptococcus agalactiae and Staphylococcus hycus were 5.65%. Comparing the overall efficacy of antimicrobials on isolate kanamycin was the most effective antibiotic where 85.7% of the total isolate were found to be susceptible.
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