జర్నల్ ఆఫ్ మాలిక్యులర్ బయోమార్కర్స్ & డయాగ్నోసిస్

Contribution of PON1 Polymorphism in Senile Cataract among Diabetic and Non-Diabetic Egyptian Patients

Abstract

Ola M. Ali, Laila Kamal Effat, Khalda Sayed Amr, Amira A. Abdel Azeem and Shaimaa M. Hassan

Background: The development of senile cataract is a multifactorial process with oxidative stress and its sequelae are clearly involved in its etiology. Cataract is also one of the earliest secondary complications of diabetes mellitus. Paraoxonase (PON) enzyme is an antioxidant High-Density Lipoprotein (HDL)-associated enzyme. In mammals, three genes of paraoxonase, PON1, PON2, and PON3, have been identified.

Aim: To assess the contribution of PON1-55 and PON1-192 gene polymorphisms as risk factors for senile cataract formation among diabeticand non-diabetic Egyptian patients.

Methods: 132 Egyptian cataract patients (66 without diabetes and 66 with diabetes) and 106 subjects with matched age and sex free of cataract and diabetes control subjects were included in the present study using multiplex PCR for PON1-55 and PON1-192 gene polymorphisms followed by restriction fragment length polymorphism analysis.

Results: The study revealed that there was a significant difference in PON1-55 genotypes; LL, LM and MM (p=0.0001) and in PON1-192 genotypes; QQ, QR and RR (p=0.0001) genotypes distribution among cataract patients with and without diabetes and controls. Also there was a significant difference in L and M (p=0.003) and in Q and R allele frequencies (p=0.005) among cataract patients with and without diabetes and controls. In addition there was a significant difference in the distribution of 55 LM/192 RR combined genotypes with the highest frequency in cataract diabetic subgroup (75%), while 55LL/192RR, 55LL/192QR and 55LM/192QR combined genotypes showed the highest frequencies among the control group (52.4%, 59.1% and 66.7% respectively).

Conclusion: For the first time, we provide evidence that functional polymorphisms in the PON1 gene may influence the risk of cataract in both non-diabetic and diabetic subgroups in Egyptian populations, suggesting new clues that help to clarify the pathogenesis of cataract.