క్యాన్సర్ సైన్స్ & థెరపీ

Discovery of Isoform p-53 Protein in Failed Cases of Chronic Lymphocytic Leukemia by Elisa Method

Abstract

Aurelian Udristioiu, Manole Cojocaru and Delia Nica-Badea

Introduction: Some diagnosed cases of chronic lymphocytic leukaemia (CLL) with possible mutations of the p-53 gene in the human B lymphocyte
genome were investigated in this study.
Method: Using the ELISA technique, the frequency of p-53 protein expression in 20 patients diagnosed with CLL, was analyzed, including the
relationship of this protein to the disease status, in the stages II-III/IV.
Results: The frequency of increased expression of the isoform p-53 protein in type B-CLL was found to be 15% per cent. The mean concentrations
of the p-53 proteins in 17 cases out of the 20, was found to be 16.76 μg/dl, with CV=0.5% and the probability index p=0.034. The percentage
of the p-53 positive isoform proteins increased above the normal values with disease progression: 15% ± 2 in stages 1-2, compared to 100% in
stages 3-4.
Conclusion: The ELISA method has proved a useful prognostic tool of CLL because was able to identify the patients with p-53 isoform proteins
and can be considered a screening method for the applying of personalized treatment in the cases diagnosticated with resistance CLL to the
specific treatment applied of the first line.