AM Elessawy, AA El-Barbary and Eman A ElBastawissy
New bromazepam derivatives namely; 7-bromo-5-(pyridin-2-yl)-1,3-dihydro-2H-benzo[e] [1,4]diazepine -2-thione and 7-bromo-1-(4,6- dichloro-1,3,5-triazin-2-yl)-5-(pyridin-2-yl)-1,3-dihydro-2H-benzo[e] [1,4]diazepin-2-one have been prepared and characterized by spectroscopic methods (FT-IR, 1H-NMR, UV-visible and EI-mass). Furthermore, the fluorometric method has been developed to detect bromazepam in biological fluid. The new methods are based on measuring excitation and emission spectra of the reaction of bromazepam with cyanuric chloride in pyridine solution compared with excitation and emission spectra free bromazepam in methanolic solution.
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