..

బయోఅనాలిసిస్ & బయోమెడిసిన్ జర్నల్

మాన్యుస్క్రిప్ట్ సమర్పించండి arrow_forward arrow_forward ..

వాల్యూమ్ 2, సమస్య 6 (2010)

పరిశోధన వ్యాసం

31 P-NMR and Differential Scanning Calorimetry Studies for Determining Vesicle’s Drug Physical State and Fraction in Alendronate Liposomes

Eyal Afergan, Yousef Najajreh, Dikla Gutman, Hila Epstein, Omar Elmalak and Gershon Golomb

Background: A liposomal delivery system requires a complete understanding of the physicochemical characteristics of the drug– liposome system in order to predict their behavior and stability in-vitro and in-vivo .

Objectives: Develop a rapid and simple experimental method to determine the fractions of the drug, alendronate (ALN), encapsulated and as a free form distributed in the liposomal suspension, and the physical state of the encapsulated drug.

Methods: 31 P-NMR measurements utilizing Ga +3 as a shifting reagent in comparison to HPLC determinations, theoretical calculations and differential scanning calorimetry (DSC) studies of various liposomal ALN formulations.

Results: The 31 P-NMR demonstrated that titrating liposomal ALN with increasing amounts of Ga +3 induced a signi fi cant shift in the exterior fraction without changing the interior fraction. Quantitative determination of the encapsulated and non-encapsulated f ractions of ALN has been achieved at Ga +3 concentrations of 3.2-25mM. The DSC study revealed that none of the formulation ingredients is in a solid phase.

Conclusions: 31 P-NMR was found to be sensitive enough to allow accurate differentiation of the distributed fractions of ALN, encapsulated and the non-encapsulated free form. Based on theoretical calculations and DSC analysis it can be concluded that AL N is dissolved in the aqueous core of the liposome.

పరిశోధన వ్యాసం

A Simple and Sensisitive HPLC Method to Monitor Serum and Synovial Fluid Concentrations of Ketorolac in Reumathologic Patients

Loretta Franceschi, Sara D’aronco and Mario Furlanut

A rapid, selective and sensitive isocratic reversed-phase HPLC assay coupled with UV detection for quanti fi cation of ketorolac in serum and synovial fl uid samples has been developed. Analytes were extracted on solid-phase cartridges (SPE) and chromatographic separation was achieved on a C18 column.

The chromatographic peak area ratio based on UV absorbency at 313 nm was used for quantitative analysis. This HPLC method has been successfully used for routine evaluation to monitor serum and synovial fl uid concentrations in reumathologic patients affering to our institute. Thanks to its sensitivity, this HPLC/UV method is also suitable for pharmacokinetic studies.

ఇండెక్స్ చేయబడింది

arrow_upward arrow_upward
https://www.olimpbase.org/1937/