మాలిక్యులర్ మరియు జెనెటిక్ మెడిసిన్

???????: ?????? ????????? ?????? ???????? ????????? ??????? ????, ?????? ????? ????-???? ??????? ???????? ??????? ?????? ????? ??????????? ???????. ?????? ?????????‌?? ????????? ????????????? ?????????????????? ????? ????? ????????? ????????????‌??? ????‌??????, ??????????? ????? ????????????? ?????? ????????? ?????????????. ???????: ?????? ????????? ??????? ????????? ????? ????????????? ?????????????????? ????? ????? ????????? ??????????? ??????????‌?? ccCK18 ????? ??????? ??????????‌?? ??????????????? ? ??????? ???????????????. ??????: ?????? ???? ??????????? ?????????‌?? 40 ?????? ????????? ?????? ????? 38 ????????? ??????????? ???????? ?????????? ???????? ?????? ???? ??????? ???????????????. ??????????? ?????, ????????? ?????? 4 ????? 6 ?????? ?????? ??????? ????? ???????? ?????? ????? ???? ???????? ???????. ???????? ????????-???????? ???????????? 18 (ccCK18)?? ?????????? ELISA ?????? ?????????????. ccCK18 ????? ?????????? ????? ????? ???????????? ????? ???? ????? ??????‌? ???? ??????? ELISA?? ???????????????. ????????? ??????????? ????????? ccCK18 ???????? ????????? ????? ????????? ??????????????? ????? ???????? ???????? ???? ???????. ??????????? ????? ????? ??????? ??????, ???????‌?????? ????? ?? ?????? ????? ?? ????? ??-?????? ????? ?????? ????????? ???? ??????? ????? ???????????? ?????????????? ????? ????????? ?????????????????. <0.05 ????? p-????? ?????????? ???????????? ??????????????. ???????: ???????? ??????? (% CI= 95%, p <0.05) ???? ?????? ????????? ???? ??????? ???????? ccCK-18 ????? ???????? ???????? ???????? ???????? ???????. ????????? (p <0.05) ?????? 6 ?????? ccCK-18 ?????? ???? ???????? ?????????. ??? ????? ?????????? ???????? pT3 ????? ??????? ???? ?????? ??????? ??????? ???????? ?????????????. ????‌???????????? ????? ???? ????? ???????????? ???? ??????? ccCK-18 ?????? ???????? ???????? ????????????. ????????? ?????? ???????? ?????????????? (LDH) ???????? ???? 6 ?????? ???????????. ??????????? ????? ????? ?????? ???????? ????? ??????? ???????? (ALT, AST, ALP ????? ?????? ??????????) ???????????, ????????? ?????? ????? ????? ????? ???????? ???????????. ????????: ??????? ccCK-18 ???????? ???????? ?????????????????? ????? ?????? ????????? ??????????? ????? ????????????? ????? ????????? ?????? ??????????‌?? ?????????????, ???????? ?????? ?????????‌???; ???????????, ??? ??????????‌??? ?????? ????????? ??????? ???????? ????????? ????????? ??????????????? ???? ???????????.

After the advent of third generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs), oncologists are called to face new challenges in everyday management of EGFR mutated non-small-cell-lungcancer (NSCLC) patients. These drugs, in particular Osimertinib (which is the only one currently available), represent an extraordinary innovation. But while raising the bar of expectations, they pose us new challenges. Mechanisms of resistance to Osimertinib are heterogeneous: from a "molecular point of view" they can be categorized in EGFR-dependent and independent ones. In recent years many clinical reports have shown interesting results with target treatments, mainly chosen on the basis of the "molecular resistance". However, in common practice clinicians and patients must face off with the reality and with limited treatment options. It may be helpful to classify different clinical patterns of disease progression during treatment with Osimertinib. Treating a localized progression to a single organ certainly differs from treating a wide dissemination of disease, as well as treating symptomatic progressions differs from treating non-symptomatic ones. This mini-review aims to analyse, with a very practical approach, current options for clinical management of EGFR mutant NSCLC patient at the time of disease progression during Osimertinib, by focusing particularly on maintenance strategies beyond progression.

Bioorthogonal chemistry and reactions developed from innovations in chemistry, having their excellent biocompatibility can be applied in various biological reactions. As these reactions, does not interfere with other reactions in the biological system, they can be used as excellent tools for tracking and studying dynamics of various biological processes. In this mini review, only the applications of bioorthogonal chemistry in host-microbe interaction and targeted therapy are discussed.

Previously, the acetylcholinesterase inhibitor Donepezil was used in a case study to address the symptoms of constipation, obstipation, and impaction in four patients with Lewy Body diseases. In patients with both Parkinson’s disease (PD) and Neurocognitive Disorder with Lewy Bodies (NCDLB), the use of Donepezil led to symptom reduction. After six months, the symptoms of the same patients were reviewed, with no loss of bowel motility nor emergence of new symptoms. After twelve months, another review of symptoms was conducted. The results indicate that Donepezil appears to be effective in reducing the symptoms of constipation, obstipation and impaction over an extended period of treatment.

Diffuse large B-cell lymphoma (DLBCL) Diffuse large B-cell lymphoma (DLBCL) is an aggressive type of non- Hodgkin lymphoma that develops from the B-cells in the lymphatic system. Under the microscope, large malignant lymphocytes are seen diffusely throughout the specimen. Diffuse Large B-Cell Lymphoma (DLBCL) accounts for 20– 30% of all lymphoid malignancies. This subtype forms distinct morphological, molecular, and phenotypic variants. Therefore, it is of a great value to classify diffuse large B-cell lymphoma according to gene expression profiling into germinal center B-cell subtype, activated B-cell subtype, and unclassified subtype. The outcomes vary between these subtypes, and the germinal center B-cell subtype has a better outcome with the current standard rituximab, cyclophosphamide, hydroxydaunorubicin, vincristine, and prednisone (R-CHOP) immunochemotherapy.

Fetal tissue transplantation is an attractive field of modern medicine that can have immense application in treating several refractile conditions. Fetal tissue transplantation is an allogeneic transplantation procedure and like any allogeneic transplantation they contribute towards the formation of a chimera at the cellular and tissue level grossly and can be classified as a macro chimerism. The fetus during the time of pregnancy also takes part in microchimerism through fetomaternal cell trafficking where there is an exchange of the maternal and the fetal cells through the blood-placental barrier. Apart from the creation of a stable chimerism, fetal tissues also play a role in healing. Fetal thymic transplantation is one of the most exciting applications of regenerative medicine which has also been discussed briefly with some case studies.

Aedes mosquito-body is the favored habitat for the member of Flaiviridae, the most famous member of the family, formerly known as Dengue virus (DENV). Dengue virus infection induces high fever in human and responsible for associated symptoms like skin rashes. In literature, it has been noted that the onset of dengue fever usually occurs in monsoon and winter seasons, which gradually declines with onset of summer. This season-coordinated trend has suggested positive association towards the climate and proliferation of Dengue virus. To investigate this hypothesis, this study has been proposed. In this study, the date-wise data for Dengue positive cases were obtained from the Government hospitals across Gujarat region. The data were further correlated with the climatic parameters for that date. The investigation suggests the strong correlation between climatic fluctuations. The correlation analysis of obtain data suggests the fluctuations in relative humidity, temperature and pressure during day and night has strong impact. We proposed Poisson regression model and Negative Binomial model for prediction.

??????? ????? ????: ????????????? ?????? (PD) ???????? ?????? ???? ??????? ????????? ???????????? ?????? ??????? ???????????? ???????????. ??????????? ?????? ????? ???????? ??????? ?????????? ??? ?????? ?????????? ????????????. ???????? ??????????? ???????????????‌?? ??????????????‌?? ??????? ?????? PD ??????? ?????? ??????? ?????? ??????????‌?? ?????????? 21 ???? PD ?????? ?????? 86 ???? ????????? ???????? ?????? ????? ???? ????????????????.
???????: ???? ???? ???? ??????, ?????? ?????????????????? ????? ??? ????? ?????? ????? ??????????? ??????????????? ???? ???????????????? ????? ??????-????? ?????? ????????????. ?????????????? ???????? ??????, ???? ?? ?????????? ?????? ????????? "???????? ????????????????" ??? ???????????, ??? ? ???? ???? ?????? ??????.
???????: PD ????? ???????????? ???? ?????? ???????? ???????? ????? ??????? ?????? ???????? ??????? ?????????, ???????? ??????????, ????????????????? ?????????. ??????????????‌?? ?????? ???? ??????? ?????? ?????? PD- ??????? ?????? ??????? ??????????‌?? ??????? ????? ?????? ????? ???? ???????????. ??? PD?? ?????????????? ????? ????? ???????????? ??????????? ???????? ????? ???????? ??????? ??????????? ????????? ??????????????. PD ????? ??????????? ????? ????? ??????? ????? ???????? '???? ??????' ????? ????????? PD ????? ???????? ????????? ?????????????? ????? ????????????‌??? ??????????? ???????? ?????????? ??????????? ????????????.

??? ??????? (?????????????) ??????? ?????? (????? ????? ???? ??????? ????? ???? ????? ?????? ???????????? ?????????. ?????? ????? ?????????? ??????? ???????? ????? ??????? ????????? ????????? ?????????????. ???????? ???? ???? ???????? ???????? ??????????'?? ?????? ???? ?????????? ????????? ????????????? ?????? 100 ???? ????????????, ??? ????????? ??????????????. ?????????? ????? ???????? ???????? ????, ??? ???????? ???????? ???????? ????????? ???????: ?????-???? ???????, ????????????, ????, ????????? (????? ??????? ???????), -????? ????????, ???????? ????? ????????? ???????? ?????? ???? ???????? ???????? ??????, ???????? ???????? ????? ????????? ??????? ????? ?????? ????‌?? ????? ????????????? ????? ???????????? ???????? ??????????????? ???????? ????????????????. ?????????? ????????? (????????? ????????????? ?????? ????????????? ???????????) ?????????, ???????? ????? ????????? ???????? ?????? ????? ???? ???????? ????? ???????????? ??????????? ??? ??????? ???????? ?????? ????????.

???????: ????? ??????????? ????????? (ASD) ????? ?? ?????? ??????‌?????? ?????????, ??? ?????? ?????? ????? ??????? ?????? ??????? ????????? ????????, ???????? ???????? ????? ??????? ????????????‌?? ????????????. ASD ?????????? ??????????. ??? ????????? ASD ???? ????? ????? ????????? ??????????????. ASD ????????? ???? ????? ???????? ????? ?????? ???????? ????????? ????.
????????: NIHM-????? ????????‌?? 3 ????? 4 (?????? n=1233 ????? n=2890) ?????????????????. ?????? ?????? ???????? ???????????????. ?????? ???????? ????????? ???? ????????? (λ ???????). Plink ????? R (ReactomePA ????? ??????????? ??????????) TDT, ?????????? ???????? ????? ??????????? ????‌?? ???????? ???? ?????????????.
??????? ????? ????: ????? ??????? “????????? ???????‌? ?????? ????? ??????” ????? “??????? ??????????????” ????????? ????, ????? ??????? “?????‌????????????? ???????????? ??????????? ????‌??” ????????? ?????????. "??????? ???????? ????‌??" ????? ??????? ????????? ???? ?? ??????? ???????. ????????? ??????? ???????? ????????????????? " NCAM1 ?????? ??????" ???? ???????? ????????? ????? ????? ???????????? .
???????: ???? ?? ???? ?????????? ????? ????-???????????? ?????????? ASD?? ?????????? ??????????? ????? ????????. ?????? ??????‌????? ????? ????????? ??????????? (T-????) ????? ? ??????????? ?????????? ????? ASD ????? ??????????????? ????????????.

????????-6-???????? ?????????????? (G6PD) ???? ????? ????????? ?????? ?????????? ?????????‌?? ???? ?????? ?????. ? ??????? ????? ???? ????????? ????? ??????????? ????? ????? ?????????? ?????????? ?????????? ?????????, ? ??????? ????? ??????? ? ?????????? ??????? ????? ?????????????? ???????????, ?????? ????????? ??????? ????? ?????? ????????? ?????????? ????????? ??????? ????? ???????????? ?????? ?????? ????? ????. . ?????? ?????? ?????? ????????? ?????? 544 ?????? ???? ???????? 16-65 ????????? ?????? ?? ???????? ????? ?????????????, ?????? 426 ???? ????????? ????? 118 ???? ?????? ????????? ???????? ????? ???????. G6PD ???? ???? ???????? ????????? ?????????? ????????? ??????? ???????????????, ??????????? ????? ????????? ??????? ?????? ?????? ??????????. G6PD ???? ????, 3.86% ???????????? ????????????, 81.48% ??????? ???????? ???????? ?????? ????????? A-202 (G> A)?? ??????????????. G6PD ???? ????? ???? ???????????? ? ??????????? ???????????? ???? ????????? ?????????? ???????? ?????? ??????? ??????????? ????? ???????????? ????????????.

????? ????????? ????????? ??????? ???? ???? ??????? ?????? ?????? ?????? ????? ?????? ??????? ????????????? ???? ??????? ???????, ????????? ????? ????? ?????????‌?? ???????? ??????? ????????? ??????????????. ??????? ?????? ?????? ??????? ???? ??????? ???????? ????? ??????? ??????? ???????, ??????? ????????????‌??? ??????????, ??????????? ???? ???????? ????? ?????? ?????????? ???????????. ???-????????????? ????????? ??????? ????-?????? ?????? ???????? ????????? ????? ????? ????? ????????? ??? ????????? ?????? ?????????. ??? ??????????????? ??????????? ????? ????? ????? ????????? ?????? ????????????????? ???? ???????????. ??????????? ?? ??????? ??????????????? ????? ????????????????? ?????????? ????? ???????????? ????? ???? ????? ??????????????? ???? ?????-??????????, ????? ??????????? ????? ????? ????? ????????????? ??????????????. ????-??????? ?????, ??????, ???? ????? ???????? ?????????, ?????? ????-??????, ???? ???? ???????? ???????? ????? ????????. ??????? ??????????‌?? ????? ?????, ????? ??????? ?????? ??????????????? ?????? ?????????? ??????????? ?????? ??????????. ??????? ??????? ???????? ??????????????. ?????????? ?????????????? ?????????? ??????????? ??????????? ????? ??????? ???? ?????? ???? ?????????? ????????????? ???????‌??? ??????????. ????-?????????, ??????????? ???????????, ???????? ????? ??????????????????? ???????, ???????????? ????? ????? ??????????? ????? ????-??????? ????? ????-???‌????????????? ????? ???????? ??????? ???? ?????? ???????? ?????? ???????. ???? ????? ????????? ??????????? ?????????‌??? ??????????????? ???????????? ?????????? ??????????, ??????? ????? ??????? ???? ?????????? ?????????? ?????????? ????????????. ???-????????????? ????? ??????? ???? ???????? ????? ??????? ??????????????? ????????????.

???????: NF1 (????????????????????, ???? 1) ??????? ????? ???????? ??? 1 ????? 2 ?????????????‌?? ??????????? ??????? ?????????‌? ???? ????? ???????? ???????????? ????????????. RNF135 (????? ?????? ???????? 135) ????? ?????? ????????, NF1 ???????, ????????? ????‌?????? ?????????‌?? ??????? ??????????????, ???????? ???????? ???? ????????? ??????? ??????????????.
???????? ????? ???????: ???? ????????-????????????? ?????????????? ???????????????? ?????? ??????????????, ???? 1 ???????????????????? ????? ???? ???????? ???? ???? ?????? ??? ?????????????? ??????????????? ????? (715K) ????????????????. ??? RNF135 ????? NF1 ????? ????? ???????‌?? ??????? ????? ???? ????? 126kb ??????? ?????????? ??????? .
???????: ????????????? ??? 1 ????? ???? 2 ?????????????‌? ???? ????? ???? 1 ????????????????????‌?? ????? ???????? RNF135 ????? ???????? ??????? ? ??????? ?????? ????????? .

??????????? ?????????? ??????? (IBDs) ?????????? ????? ????????? ????????? ?????????? ?????????? ????? ????? ???? ???????? ?????????? ????? ????? ?????. ?????? ????????? ??????‌???? ???????? ??????? ?????? ????????? ??????????? ???????????, ????, ???? ??????????, ? ???????? ?????? ?????????? ???? ???????????? ?????. ???????????, ???? ????? ????? ?????????? ????? ?????? ????? ??????? ???????? ???????????. ???????? ?????? ???????? ?????????? IBD ????? ????????? ????????????? ??????? ?????? ???????, ????????? ????????????? ??????????? ????? ????? ?????? ???????????????. ?????? ????, ?????? ??????????? ????? ???????? ?????? ?????????? ?????? ??????‌???? ????????? ????????? ??? ???? ????? ??????? ???????? ???????? ?????? ?????????????‌?? ??????????. ??-???????? ????????????? (HTS) ??????????? ???? ?????? ????? ?????? ?????????? ??????????? ???????? ??????, ???????? ??? ???? ???????? ????? ?????, ????????? ????? ?????? ????????? ????????????. HTS ?????????? ???? ??????????? ????? ?????? ????????? ??????????‌?? IBD? ?????? ????????? ?????? ????????????????.

??????? ???????? ???????? (AML) ????? ??????????? ????????????? ?????????? ????? ?????? ?????? ??????? ????. ???????? ????????? ?????? ??????-??????? ????? ???? ?????????? ???? ????? ??????? ??? ?????? ?????? AML ??????? ?????? ???????, ??? ?????? AML ????? ????? CD8+ T ???? ????????? ???????? ????????????. ?????, ???? ?? ???? ????? ??? ?????? ??????? AML???? ????????-??????? ????‌???????‌? ?????????? ????? CD8+ T ???? ????????? ????? ?????? ???????? ????? ??? ????????? ????‌???????‌? ????????????? ??????? ???? AML ????????? ????? ???????????.

???????: ?????? ????? ????? ????????? ?????????? ?? ????? ?????? ????? ????? ???????????? ????. ???? ????‌???? ????? ?????? ?????????? ????? Google ???????? ????? ????? ????‌????? ???????? (SNA) ????????? ????????? ????????????‌??? ???????? ???????????.
????????: ??????????? (???????) ????? ???????? (???????) ????? ???? ?????? ????‌???? ??????? ????? ???????? 13, 2017? 6,732 ?????????? ????‌???? ????????????, ???? ? ??????? ????????? ???????????: (1) ???? ????? ?????? ??????; (2) ???????? ?????? ???????????? ?????? ?????????; (3) SNA???? ?????? ????? ????? ????? ???????. ???? ?????? ??????????????? Microsoft Excel VBA?? ?????????? ??????. ?????? ????? ????? ????????? ???????? ???????????????? Google ???????? ????? SNA ????? ???????????????.
???????: (1) ??????? ??????? ?????? US (1622,31.88%), ???? (361, 7.10%), ????? ????? (356, 7.00%) ????? ?????????? ???? ???????????;(2) ??????? ??????? ??????‌?? ???????? (103, 1.53%); (3) RT-PCR ????? ???????? ?????????? ????? ????? ?????????? ??????? ???????? ???????????‌?? ????????????; (4) ??????? ???????? ??????????? ???? ????????? ?????? ?????? ?? ?????????.
???????: ????? ????‌????? ???????? ???????? ?????? ???? ????????? ??????? ????? ????? ????????????? ???????????. Google ???????? ?????? ????? ??????? ???????????? ????????? ?????????? ???? ???????? ????????????.

???????:   ???????? ??????? ????? ??????? ????? ????????? ?????? ????? ????? ??????????? ????????????? ???????. subsp. ????????? ??????. var sipyleus L. (?????? ????? ??????? ????? ????????????????), ??????? ??????? L. ????? ???????? ??????? L. ?????? ????? ?????? ????????? ?????? ??????.
????????: ???? ????? ????? ?????? ????? ?????????? spp., E. ????, ???????????? ????????, ?????????? ?????????, MSSA, ???????????? ?????????? ????? ????????? ??????????‌??? ???? ??????. ????? ????? ????? ??????????? ???? ?????? ????? ?????? ????????? ?????? ?????? ????? ?????????.
???????: ????????? ?????????? ???????? ??????? ??????????? ?????? ???? ???????? ????????????, ?????? ?????????????? ?????????? ????? ???????????? ???????? ???????. ?????????? ????????? ? ?????? ??????????? ???????.
???????: T. ?????????, O. ??????? ????? S. ??????? ????? ???????? ?????? ???????? ?????????? spp ???? ?????? ??????? ??????????? ???? ???????????? ???????. ????? ???????????? ??????????. ? ??????? ???????? ??????? ????? ??????? ??????? ????? ???????? ??????? ?????? ??????????????? ???? ????????????. ???????????? ???????????? ?????????? ???????????? ??????? ?????????? ? ??????? ????????????????? ???? ??????????????.

???????????: ???? ??????? ???????? ???????????, ???????? ?????? ????? ???????????/?????????? ??????????? ?????? ??????? ????. ??? ???????????, ???? ?????? ??????? ?????????? ????????????? ????????? ?????????? ????? ???????? ???????? ????? ???????? ??????. ???? ????????? ????????????-??-????????? ????????? ????? ???‌????????? ???‌???????? ?????????? ???? ???? ???? ?????? ????????? ?????????? ???????????. ????????? ??????? ????? ?????‌??? ? ????? ???????? ????????? ??????????? ???? ?? ????????? ??????? ??????.
????????? ????? ????????: ???? ??????? ??????????? ??????????. ???? ????????? ???????? ?? ???????? ????? ???????? ????? ????? ????? ?????? ????????? ??????????. ????? ???????????? ????????????-??-????????? ????????? ????? ???? ???? ???? ?????? ??????? ????? ?????????? ????? ?????????? ??????? ????????. 80% ????? (????????? ??????=3.2)?? 20% ????????????? ???????????? ????? ????????? 130 ????? ??????? ?????. 0.05 ???? ?????? p-??????? ???????????? ???????????????.
???????: ???????? ??? ??????? ???? ???????? ?????? 32.4 ± 4.1 ??????? 137 ???? ?????? ??????? ????? ??????? ??????? 39.2 ± 1.1 ????? ???? ???????? ?????? ?????? 145 ???? ?????? ???????. ??????? ?????? ??????????????-????????? ????????? ??????? ??????? ??????? ???????? ???????? ???????????? (5.9 ± 5.1 vs. 4.6 ± 3.2, p=0.007).
??????? ?????? ???? ???? ???? ?????? ??????? ?????? ???? ???? ???? (13.6 ± 0.9, 13.9 ± 1.8, p=0.09). ?????????‌? ???????? ???? ????? (n=52) ???? ???????? ?????? <35 ?????? (n=72) ???? ???????? ????? ?? ???? ???????? ????? ??????? ????????????? ???????? ?????? ???????.
???????: ????? ?????????? ????????????? ??????? ??????? ??????? ????????????-??-????????? ????????? ???????? ?????????. ?????? ???? ???? ???? ???? ?????? ????????? ??????????? ???????? ???? ????????????.