Lenimin Xenia
The field of functional genomics has witnessed a remarkable transformation in recent years with the advent of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology. Among the various applications of CRISPR, loss-of-function libraries have emerged as powerful tools for systematically interrogating gene function on a genome-wide scale. This article provides an in-depth exploration of CRISPR loss-offunction libraries, their development, applications, challenges, and the transformative impact they have had on advancing our understanding of genomics.
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