బయో ఇంజినీరింగ్ & బయోమెడికల్ సైన్స్ జర్నల్

Immobilization of Glucose Oxidase in Anthracene-Based Semi-Conducting Polymer: Application on Glucose Biosensing

Abstract

Dhekra A, Khaled H, Mina S, Mustapha M and Ali O

A glucose biosensor based on the immobilization of the enzyme glucose oxidase (GOx) onto an anthracene-based polymer analogue of poly (phenylene sulfide) (PAnS)/Gold electrode was elaborated. The building of the bridge system was evaluated by electrochemical impedance spectroscopy measurement (EIS) and cyclic voltammetry (CV). The PAnS semi-conducting polymer exhibits a good film forming, stability and adhesion on gold Electrode. The choice of the immobilization of GOx in thin film polymer is essentially based on the facility of the synthesis of the polymer also on the surface properties. The immobilized GOx was proved by cyclic voltammetry, contact angle and UV-visible spectroscopy. The GOx/PAnS/Au bioelectrode showed a linear response to glucose in the concentration ranging from 10-8 M to 10-3 M with a correlation coefficient of 0.98 and a sensitivity of 2.66 (kΩ M-1cm-2). Indeed, The GOx/PAnS/Au structure could make it a promising bioelectrode for detection of glucose in the biological samples.